Protein Details

-Genomic Data for HLH-27			Links
Gene hlh-27	Species $species	Domain bHLH	IHOP 47526	Swiss-Prot Q18056
UniPROBE Accession Number $accession_id	Synonyms None Available		$refseq_designator NP_495065	Wormbase Protein ID WP:CE04030
UniPROBE Accession Number $accession_id	Synonyms None Available		EdgeDB Promoter Phlh-27	Wormbase Gene ID WBGene00001966
Description (from Wormbase) hlh-27 encodes a REF-1-like protein with two bHLH domains, that represses lag-2 transcription in ABa descendants (in response to a Notch signal, redundantly with REF-1, HLH-25, HLH-26, HLH-29, and perhaps HLH-28) and tbx-37 transcription in EMS descendants (independently of Notch, redundantly with REF-1, HLH-26, HLH-29, and perhaps HLH-28); lag-2 repression by HLH-27 may require UNC-37; HLH-27's paralogs include HLH-25 through HLH-29 and (more distantly) REF-1; hlh-27 is transcribed in the early MS lineage, probably due to MED-1/2 binding of clustered sites in the hlh-27 promoter region; HLH-27 is expressed in ABp granddaughters ~25 minutes after ABp first contacts an APX-1-expressing cell, and in ABa descendants after a second Notch interaction; HLH-27 is also expressed in four EMS granddaughters (MSaa, MSap, MSpa, MSpp) beginning at the 24-cell stage, in a SKN-1-dependent manner; HLH-27 is required for embryonic viability in mass RNAi assays.

-PBM Motif Data for HLH-27
PBM-Derived DNA Binding Site Motif (Seed-And-Wobble)				PWM View Save
PBM-Derived DNA Binding Site Motif (Seed-And-Wobble)				Top Kmer C.CGTGTCC E.S. 0.498 GGACACG.G E.S. 0.498
Downloads Download the zip of all HLH-27 PBM files or view the downloads directory for individual files.

-HLH-27 Datasets
Gene	Homodimerizes	Clone Type	Last Modified	Save	View
hlh-27	Yes	Not full length	2014-07-16
HLH-27 Insert Sequence `1 PKVIPSSMSD YRSVPYNQTP KSGSERKRRN ITNELINECK TIVQKSEEEH 51 ISQEVVLFRI VKLVTGVNLE SNFSSNDLSE STRRKFDTES ERRKVKTERE 101 KIRRKKQDDC YAELKFFILN KQMGSYEQRL KLERITILEI IIDYIKHNSD 151 LLYPETIPQI LPLLAGKSTA TCENKENEKP KTRMEVKDLF PRLTFQEVQE 201 SPTSTSPLLT FPCIPMIPTT QFNVLSNYNT VPSIFSAPLR FILPSLQILT 251 PETSDEEENE ETVDISN` Mutations away from WormBase HLH-27 wildtype: • Methioinine start not included in insert sequence